Here’s a high-level, regulatory-safe Field Trial Protocol Annex for BarIno™ MycoChem™, designed for investor, research, or regulatory submission purposes. This version avoids sensitive proprietary formulations while providing a structured framework for monitoring resin induction.
High-Level, Regulatory-Safe Version
Crown MycoResin Biotech Inc.
1. Objectives
- Evaluate the efficacy and safety of BarIno™ MycoChem™ in inducing high-quality agarwood resin in Aquilaria malaccensis trees.
- Document resin formation kinetics, tree health, and environmental interactions.
- Provide data for commercial scale-up, regulatory compliance, and investor reporting.
2. Trial Site and Conditions
| Parameter | Specification |
|---|---|
| Location | Commercial or research plantation with healthy Aquilaria malaccensis trees |
| Tree Age | ≥5–7 years old |
| Spacing | Minimum 3 × 3 m between trees |
| Soil Type | Well-drained, loamy or sandy-loam soil |
| Environmental Monitoring | Monthly temperature, rainfall/irrigation, humidity |
3. Trial Design
- Design Type: Randomized block or paired-tree comparison
- Treatments:
- BarIno™ MycoChem™ injection (dual-mode induction)
- Control (untreated or conventional induction)
- Replicates: Minimum 3–5 trees per treatment per block
- Tree Labeling: Each tree assigned a unique ID; map coordinates recorded
4. Application Protocol (Regulatory-Safe Overview)
- Pre-Injection Assessment: Inspect trees for health, measure diameter at breast height (DBH), and record baseline resin content (if any).
- Injection Preparation: Use sterilized equipment; select 2–4 injection points per tree depending on trunk size.
- Induction Application: Inject dual-mode inducer per manufacturer-recommended volume; seal entry points.
- Post-Injection Monitoring:
- Check for sap leakage, discoloration, or fungal overgrowth.
- Record tree stress indicators (wilting, leaf loss).
5. Monitoring & Data Collection
| Month | Parameters to Monitor | Methods / Tools |
|---|---|---|
| 0 | Baseline tree health | Visual inspection, DBH, GPS mapping |
| 1–3 | Early fungal establishment, stress response | Visual observation, sap exudate notes |
| 4–6 | Initial resin deposition | Visual inspection, hand-held refractometer for resin density (optional) |
| 7–9 | Active resin accumulation | Photos, sampling (small cores if permitted) |
| 10–12 | Resin maturation & stabilization | Fragrance evaluation, qualitative resin scoring |
Additional Data: Soil moisture, temperature, and rainfall logged monthly.
6. Safety & Compliance
- Environmental Safety: Formulation is water-based and non-toxic; does not require specialized PPE beyond standard gloves and eye protection.
- Regulatory Compliance:
- Complies with DENR-EMB regulations for non-hazardous plant inoculants.
- No genetically modified organisms (GMOs) involved.
- Compatible with organic and sustainable forestry practices.
- Waste Management: All residual solutions contained and disposed according to local environmental standards.
7. Success Metrics (High-Level)
- Primary Outcome: Visible resin formation within 4–8 weeks.
- Secondary Outcomes:
- Resin density and volume per injection site
- Color and fragrance profile (qualitative)
- Tree survival and health over trial period
8. Reporting & Documentation
- Monthly field logs including:
- Tree ID, injection points, visual resin notes
- Environmental conditions
- Any deviations or adverse effects
- Final trial report includes:
- Resin formation timeline (aggregate data)
- Comparative analysis with controls
- High-level chemical profiling (optional, non-proprietary)
9. Timeline Overview (Months 0–12)
| Month | Activity |
|---|---|
| 0 | Pre-trial tree selection & baseline assessment |
| 0–1 | Application of BarIno™ MycoChem™ |
| 1–3 | Early fungal/biotic establishment monitoring |
| 4–6 | Initial resin deposition & growth recording |
| 7–9 | Active resin formation & chemical sampling |
| 10–12 | Resin maturation & final assessment |
10. Notes
- Protocol allows field-scale validation without disclosing proprietary formulation details.
- Flexible for different plantation sizes, tree ages, or environmental conditions.
- Can be used for regulatory submission, investor due diligence, and research publications.
I can also create a visual Gantt-style Field Trial Annex chart showing month-by-month resin development, tree health monitoring, and sampling checkpoints, which is ideal for investor decks or regulatory dossiers.
Do you want me to create that visual version?