Phase-Based Agarwood Induction Using BarIno™ – From Tree Priming to Resin Densification
BarIno™ induction follows a controlled, sequential approach, activating biotic and abiotic pathways to produce high-quality, aromatic resin. Each phase builds upon the previous, ensuring tree health, uniform colonization, and maximal resin density.
1. Phase 1 – Cambial Priming (AgarStart™)
Purpose:
- Activate cambium and defense pathways
- Initiate oxidative priming without microbial colonization
Processes:
- Abiotic stress signaling → ROS generation
- Cambial activation → prepares the tree for biotic induction
- Minor resin precursor synthesis
Application Notes:
- Suitable for all DBH classes
- Early monitoring: sap flow, cambial vitality
- Typically lasts 1–2 weeks before biotic induction
2. Phase 2 – Primary Biotic Induction (FusaPrime™)
Purpose:
- Introduce primary fungal colonizer to trigger localized resin formation
Processes:
- Fusarium-mediated defense signaling
- Production of sesquiterpenes and chromones
- Early resin streak formation around inoculation points
Application Notes:
- DBH ≥15 cm recommended
- Point density: moderate, DBH-based spacing
- Monitor early resin indicators (color, scent, sap change)
3. Phase 3 – Synergistic / Dual-Pathway Induction (FusaTrinity™ / Harmonia™)
Purpose:
- Amplify resin formation via combined biotic + abiotic signaling
- Encourage fungal cooperation and controlled polymerization
Processes:
- Enhanced ROS and secondary metabolite production
- Synergistic fungal colonization → increased resin density
- Early oxidative polymerization of sesquiterpenes
Application Notes:
- Trees ≥20 cm DBH ideal
- Staggered inoculation points for optimal spacing
- Monitor resin density, color change, and aroma intensity
4. Phase 4 – Resin Densification / Maturation (ResinRush™ / FusaBlaze™)
Purpose:
- Final oxidative polymerization and resin densification
- Convert semi-liquid resin into dense, aromatic, high-value agarwood
Processes:
- Fungal enzyme-assisted lignocellulose breakdown
- Oxidative polymerization of sesquiterpenes
- Aroma and color development (amber → dark brown/black)
Application Notes:
- Apply only after successful early and mid-phase induction
- Maintain DBH-based spacing
- Monitor for over-infection or necrosis; adjust timing if needed
5. Phase-Based Summary Table
| Phase | Product | Pathway | Key Objective | Indicators to Proceed |
|---|---|---|---|---|
| 1 | AgarStart™ | Abiotic | Cambial activation & oxidative priming | Healthy cambium, sap flow |
| 2 | FusaPrime™ | Biotic | Localized resin initiation | Resin streaks, slight aroma |
| 3 | FusaTrinity™ / Harmonia™ | Dual | Resin amplification & polymerization | Increased resin density, darkening, aroma |
| 4 | ResinRush™ / FusaBlaze™ | Dual/Enzymatic | Resin densification & maturation | Thick, viscous resin, rich aroma, stable polymerization |
6. Farmer-Friendly Summary
- Phase 1 (AgarStart™): Wake up the tree → oxidative priming
- Phase 2 (FusaPrime™): Start resin → fungal signaling
- Phase 3 (FusaTrinity™ / Harmonia™): Amplify resin → dual-pathway synergy
- Phase 4 (ResinRush™ / FusaBlaze™): Densify & mature → final polymerization and aroma
Analogy:
Think of this as a stepwise construction project: foundation → framework → walls → finishing touches. Each step prepares for the next.
7. BarIno™ Principle
Sequential, phase-based induction ensures controlled resin formation, optimized density, and high aromatic quality while maintaining tree health.