Sequential Inoculation Protocol System

BarIno™ Sequential Resin Induction Protocol (From Tree Activation to Resin Maturation)

Objective: To induce controlled, high-density agarwood resin formation using a staged biological → synergistic → finishing system, minimizing tree mortality while maximizing resin quality.

PHASE 1: TREE ACTIVATION (Biological Entry Phase)

1. AgarStart™

Purpose: Tree immune priming & wound conditioning
When to use:

  • Tree age: 3–5 years
  • DBH: ≥ 8–10 cm
  • Healthy, actively growing trees

Action:

  • Creates safe micro-wounds
  • Activates the tree’s defense signaling
  • Prepares vascular tissues for fungal colonization

Application:

  • Drill shallow holes (1–1.5 cm depth)
  • Inject AgarStart™ at low dose
  • Seal holes properly

🕒 Waiting period: 14–21 days

PHASE 2: PRIMARY BIOLOGICAL INDUCTION

2. FusaPrime™ / MycoResin Harmonia™

Purpose: Initial resin-triggering fungal colonization
Biology: Mild Fusarium-based consortium

What it does:

  • Establishes controlled infection
  • Initiates localized resin production
  • Minimizes aggressive tissue decay

Application:

  • Inject into same or adjacent holes
  • Moderate hole density based on DBH
  • Seal all entry points

🕒 Waiting period: 30–45 days

PHASE 3: SYNERGISTIC RESIN AMPLIFICATION

3. FusaTrinity™ / MycoChem™

Purpose: Multi-pathway resin stimulation
Biology: Dual-action fungal + mineral catalyst system

What it does:

  • Expands resin zone vertically & radially
  • Strengthens resin chemistry
  • Increases aromatic complexity

Application:

  • Increase hole count slightly
  • Apply evenly around trunk
  • Avoid over-concentration on one side

🕒 Waiting period: 45–60 days

PHASE 4: INTENSIVE RESIN DENSIFICATION

4. FusaBlaze™

Purpose: High-intensity resin build-up
Biology: Advanced stress-responsive fungal blend

What it does:

  • Drives deep resin saturation
  • Darkens wood fibers
  • Boosts oil precursor compounds

Tree must be healthy before use

Application:

  • Used only on DBH ≥ 15 cm
  • Controlled spacing between holes
  • Monitor tree response after 30 days

🕒 Waiting period: 60–90 days

PHASE 5: RESIN MATURATION & FIXING (Finishing Phase)

5. ResinRush™ (Finishing Inoculant)

Purpose: Resin stabilization & oil maturation
Type: Abiotic / Myco-Chemical finisher

What it does:

  • Locks resin into wood fibers
  • Improves oil viscosity & scent depth
  • Reduces resin volatilization loss

Application:

  • Low-volume injection
  • Fewer holes than biological phases
  • Applied once only

🕒 Maturation period: 3–6 months minimum

MONITORING & QUALITY CHECKPOINTS

StageWhat to Check
30 daysResin streak formation
60 daysColor darkening
90 daysAroma development
6 monthsOil density & uniformity

HARVEST READINESS (Indicative)

  • Resin visible across multiple layers
  • Wood emits distinct agar aroma when cut
  • Core remains structurally intact
  • Oil-grade or incense-grade decision made

KEY ADVANTAGES OF THIS SEQUENTIAL SYSTEM

  • Controlled infection (low mortality)
  • Progressive resin depth
  • Consistent quality across trees
  • Compatible with contract inoculation & revenue sharing
  • Scientifically aligned with dual induction principles