6.2 Cooperative vs competitive colonization

Cooperative vs Competitive Colonization in Agarwood – Understanding Fungal Interactions for Optimized Resin Production

The success of agarwood resin induction depends not only on which fungi are introduced, but also on how fungal species interact within the tree. Colonization can be cooperative (synergistic) or competitive (antagonistic).

1. Cooperative Colonization

Definition: Two or more fungal species work synergistically, enhancing each other’s growth and metabolite production.

Characteristics:

  • Early fungi create microenvironments favorable for secondary fungi
  • Metabolites from one fungus stimulate sesquiterpene production or activate defense pathways
  • Resin formation is uniform, dense, and aromatic

Example in BarIno™:

  • FusaPrime™ (primary colonizer) → FusaTrinity™ or Harmonia™ (secondary, dual-action)
  • Result: enhanced resin polymerization and aroma

Outcome:

  • Predictable, high-quality resin
  • Controlled colonization zones
  • Minimal tissue necrosis

2. Competitive Colonization

Definition: Fungal species compete for resources or space, often inhibiting each other.

Characteristics:

  • Overlapping inoculation points or high-density fungal load
  • Aggressive fungi dominate, producing toxins that damage cambium
  • Inconsistent resin formation, patchy or necrotic zones

Risk Factors:

  • Incorrect sequencing of fungal inoculants
  • Excessive point density or overlapping inoculation zones
  • Poor tree health or stressed cambium

Outcome:

  • Necrosis or dieback
  • Uneven resin distribution
  • Reduced oil content and aromatic quality

3. BarIno™ Management Strategies

A. Sequential Inoculation

  • Mimic natural fungal succession: early colonizers → amplification → densification
  • Avoid simultaneous high-density inoculation of multiple species

B. Spacing & DBH Optimization

  • Maintain DBH-based spacing to prevent competition between fungal colonies
  • Stagger inoculation points along trunk circumference and height

C. Controlled Dual-Pathway Products

  • FusaTrinity™ or MycoChem™ combine abiotic priming and cooperative fungal signaling
  • Avoid excessive biotic load in one phase to reduce competitive inhibition

D. Monitoring

  • Early detection of patchy resin, cambium necrosis, or unusual sap flow
  • Intervene by removing over-colonized points or adjusting next inoculation phase

4. Farmer-Friendly Summary

Interaction TypeEffect on ResinManagement Tip
CooperativeDense, uniform, aromaticSequence inoculants; use BarIno™ dual-pathway products
CompetitivePatchy, necrotic, low-qualityMaintain spacing, stagger inoculations, monitor tree response

Analogy:

Cooperative fungi are like a well-orchestrated team, building high-quality resin; competitive fungi are like rivals fighting over resources, causing patchy or damaged resin.

5. BarIno™ Principle

Controlled fungal cooperation drives high-quality, dense resin.
Sequencing, spacing, and monitoring prevent competitive interactions that reduce yield and quality.