Rice + Coconut Juice Method

Below is a farmer-friendly, step-by-step SOP for the Rice + Coconut Juice Method, written in simple language, safe for on-farm use, and aligned with Barino™ agarwood inoculant practices under Crown MycoResin Biotech Inc.

This can be used as:

  • A training handout
  • A cooperative SOP
  • A TESDA community module
  • A field poster (easy to convert to infographic)

FARMER SOP

Rice + Coconut Juice Method

For Making Agarwood Fungal Inoculant

WHAT IS THIS METHOD?

This method uses:

  • Rice → body & growth space for fungus
  • Coconut juice (coconut water) → food & energy booster

Together, they help agarwood fungi grow faster and stronger for field use.

IMPORTANT RULES (READ FIRST)

✅ Use fresh coconut juice only
✅ Work clean and careful
❌ Never use sour or old coconut juice
❌ Do not open containers while growing
❌ Do not mix different fungi

MATERIALS NEEDED

Main Materials

  • Rice (white or brown)
  • Fresh coconut juice (young coconut preferred)
  • Clean water
  • Clean fungal starter (from trusted supplier)

Tools

  • Pressure cooker or steamer
  • Glass bottles or heat-safe plastic bags
  • Cotton or clean cloth + rubber band
  • Knife or spoon
  • Alcohol (70%)

STEP 1: PREPARE RICE + COCONUT JUICE

  1. Wash rice until water is clear
  2. Soak rice for 30 minutes, then drain
  3. Mix cooking liquid:
    • 50% clean water
    • 50% fresh coconut juice
  4. Cook rice until:
    • Cooked but not soggy
    • Grains are separate

💡 Rice must not be wet or sticky

STEP 2: PACK & STERILIZE

  1. Fill bottles or bags half-full only
  2. Cover opening with:
    • Cotton plug, or
    • Clean cloth tied tightly
  3. Sterilize:
    • Pressure cooker: 30–45 minutes
    • Steamer: 1 hour
  4. Let cool completely

⚠️ Do NOT open while hot

STEP 3: ADD THE FUNGUS (CRITICAL STEP)

  1. Clean hands and tools with alcohol
  2. Work in clean room (no wind, no dust)
  3. Cut 5–10 small pieces of fungal starter
  4. Put pieces into rice container
  5. Close immediately
  6. Shake gently

🚫 Do NOT touch fungus with bare hands

STEP 4: INCUBATION (GROWING TIME)

  1. Place containers in:
    • Dark area
    • Room temperature
  2. Do NOT open
  3. Check outside of container every 2 days

⏳ Growing time: 7–12 days

HOW TO KNOW IT IS READY

✅ Rice fully covered with white fungus
✅ Clean smell (mushroom-like)
❌ THROW AWAY if:

  • Green or black mold appears
  • Slimy texture
  • Bad or sour smell

STEP 5: USE OR STORE

Ways to Use

  • Use directly for agarwood inoculation
  • Mix with clean water to make liquid inoculant

Storage

  • Best used within 2–4 weeks
  • Store in cool, shaded place
  • Do NOT freeze

COMMON FARMER MISTAKES (AVOID THESE)

❌ Too much coconut juice
❌ Rice too wet
❌ Opening container during growth
❌ Poor cleaning
❌ Using expired coconut juice

SAFETY & CLEANLINESS

✔ Wash hands
✔ Clean tools with alcohol
✔ Keep away from children & animals
✔ Burn or bury spoiled cultures

WHY THIS METHOD IS GOOD FOR FARMERS

🌱 Faster fungal growth
🌱 Stronger inoculant
🌱 Low cost
🌱 Uses local materials
🌱 Easy to repeat

BEST PRACTICE TIP

Always get your fungal starter from a trusted and tested source,
such as Crown MycoResin Biotech Inc. – Barino™

If you want next, I can:
✅ Turn this into a 1-page infographic poster
✅ Translate into Filipino / Tagalog
✅ Create a Do & Don’t wall chart
✅ Adapt this SOP for cooperative-level production

Just tell me what you’d like to prepare next 🌱