Field Trial Protocol Annex (High-Level, Regulatory Safe)
Purpose: To provide a structured framework for evaluating the performance of BarIno™ MycoResin Harmonia™ in agarwood (Aquilaria spp.) without disclosing confidential formulation details.
I. OBJECTIVES
- Assess resin initiation and development under controlled field conditions.
- Monitor tree health and stress response post-inoculation.
- Provide data for sustainable induction protocols and regulatory submissions.
II. TRIAL SITE SELECTION
- Mature Aquilaria plantation or agroforestry site
- Trees ≥7–10 years old, with DBH ≥18–20 cm
- Healthy, actively growing trees
- Ensure proper spacing and environmental monitoring
III. TREATMENT DESIGN (HIGH-LEVEL)
| Parameter | Guidance |
|---|---|
| Product | BarIno™ MycoResin Harmonia™ (quad-consortium inoculant) |
| Dose | Applied per tree according to internal SOP (exact concentration confidential) |
| Application Method | Controlled trunk inoculation; ensure minimal wounding |
| Timing | Staged application across growing season; adjust to local rainfall and temperature |
| Tree Age | Minimum 7 years; optimal 7–15 years for response evaluation |
| Replication | Minimum of 3–5 trees per treatment block for statistical relevance |
| Control | Untreated trees or placebo (carrier only) |
Exact dose, volume, and inoculation procedure are proprietary and restricted to internal use.
IV. MONITORING AND DATA COLLECTION
- Tree Health: Leaf turgor, canopy density, disease symptoms
- Resin Formation: Visual assessment, resin sampling at defined intervals
- Growth Metrics: DBH, height, branch vigor
- Environmental Parameters: Soil moisture, temperature, humidity
V. EVALUATION PERIOD
- Short-term: 3–6 months post-application (initial resin initiation)
- Medium-term: 6–12 months (resin density and color development)
- Long-term: 12–24 months (aromatic profile and commercial-grade resin yield)
VI. DATA MANAGEMENT
- Collect and record observations in standardized trial sheets
- Ensure confidentiality of proprietary formulation details
- Only aggregate and anonymized data shared externally
VII. SAFETY AND ENVIRONMENTAL CONSIDERATIONS
- Wear standard PPE during inoculation
- Avoid contamination of soil and water sources
- Do not exceed recommended application frequency or dose (internal SOP)
- Monitor for unintended stress indicators
VIII. REPORTING AND REGULATORY USE
- Data used to support DENR / DA-BPI registration, field efficacy claims, and internal SOP validation
- Maintain separation of proprietary formulation information from regulatory submissions
- Include references to Confidential Internal Formulation Sheet as needed
IX. NOTES
- This protocol is high-level and regulatory-safe; detailed inoculation steps and strain ratios remain confidential.
- Modifications may be made based on local conditions, tree response, and expert agronomist guidance.
End of Field Trial Protocol Annex