Timing of Inoculation

Here’s a farmer-friendly, training-ready guide on timing and density of inoculation for agarwood trees using the BarIno Inoculation System. Proper timing and inoculation density are critical for optimal resin induction.

Timing of Inoculation

1. Seasonal Considerations

  • Best during active growth season (spring to early summer)
    • Trees have higher sap flow → better distribution of inoculants
  • Avoid dormant or stress periods (dry season, heavy rain, or frost)
    • Stress can reduce resin response or harm the tree

Farmer Tip: Trees respond faster when actively growing and healthy.

2. Tree Age

  • Recommended age: ≥5–7 years
  • Younger trees may not respond efficiently
  • Older trees may require careful inoculation to prevent damage

3. Time After Pre-Treatment

  • If using AgarStart™ as pre-treatment:
    • Apply biotic or dual inoculants 2–4 weeks after AgarStart™
  • This allows the tree to prime its defense system

Density of Inoculation

1. Number of Inoculation Points

  • Depends on tree size / DBH:Trunk DBHNumber of Points10–15 cm3–5 points16–25 cm5–8 points26–35 cm8–12 points>35 cm12–16 points
  • Use even spacing around trunk
  • Avoid clustering points to prevent localized stress

2. Depth and Volume

  • Depth: 2–5 cm into xylem (depending on tree size)
  • Volume per point: Small, controlled amounts (e.g., 5–15 mL)
  • Too shallow → low resin response
  • Too deep / too much → tissue damage, tree stress

3. Frequency

  • Typically one inoculation per season for single-use inoculants
  • Dual systems may allow repeat application after 3–6 months
  • Avoid over-inoculation → reduces survival and resin quality

4. Distribution Pattern

  • Evenly distributed around circumference of trunk
  • Avoid top-heavy or bottom-heavy placement
  • Maintain safe distance from main roots and structural defects

Farmer Reminder

“Right time + right spacing = higher resin yield, healthier trees.”

Optional Quick Guide

Example:

  • Tree: 20 cm DBH, healthy canopy, active growth
  • Inoculation: 6 points evenly around trunk
  • Depth: 3 cm, 10 mL each point
  • Timing: Early spring, 2 weeks after AgarStart™
  • Follow-up: Monitor resin formation monthly

If you want, I can next make a visual “Timing & Density” diagram showing:

  • Tree trunk with inoculation points
  • Seasonal timing indicator
  • Depth and volume for each point

This is perfect for farmer field manuals or training slides.

Do you want me to create that diagram?