1. Seasonal Considerations
- Best during active growth season (spring to early summer)
- Trees have higher sap flow → better distribution of inoculants
- Avoid dormant or stress periods (dry season, heavy rain, or frost)
- Stress can reduce resin response or harm the tree
Farmer Tip: Trees respond faster when actively growing and healthy.
2. Tree Age
- Recommended age: ≥5–7 years
- Younger trees may not respond efficiently
- Older trees may require careful inoculation to prevent damage
3. Time After Pre-Treatment
- If using AgarStart™ as pre-treatment:
- Apply biotic or dual inoculants 2–4 weeks after AgarStart™
- This allows the tree to prime its defense system
Density of Inoculation
1. Number of Inoculation Points
- Depends on tree size / DBH:Trunk DBHNumber of Points10–15 cm3–5 points16–25 cm5–8 points26–35 cm8–12 points>35 cm12–16 points
- Use even spacing around trunk
- Avoid clustering points to prevent localized stress
2. Depth and Volume
- Depth: 2–5 cm into xylem (depending on tree size)
- Volume per point: Small, controlled amounts (e.g., 5–15 mL)
- Too shallow → low resin response
- Too deep / too much → tissue damage, tree stress
3. Frequency
- Typically one inoculation per season for single-use inoculants
- Dual systems may allow repeat application after 3–6 months
- Avoid over-inoculation → reduces survival and resin quality
4. Distribution Pattern
- Evenly distributed around circumference of trunk
- Avoid top-heavy or bottom-heavy placement
- Maintain safe distance from main roots and structural defects
Farmer Reminder
“Right time + right spacing = higher resin yield, healthier trees.”
Optional Quick Guide
Example:
- Tree: 20 cm DBH, healthy canopy, active growth
- Inoculation: 6 points evenly around trunk
- Depth: 3 cm, 10 mL each point
- Timing: Early spring, 2 weeks after AgarStart™
- Follow-up: Monitor resin formation monthly