1. Timing of Application
- Apply after chemical priming: 1–2 weeks after AgarStart™ / FerroBoost™
- Apply alongside or just before biotic inoculants (FusaPrime™, Harmonia™) for best synergy
- Avoid extremely wet or dry periods to prevent enzyme washout or inactivation
Tip: Enzymes work best in actively growing trees with moist tissues, not in dormant or stressed trees.
2. Inoculation Points & Density
- Number of points: Follow tree DBH guidelines (3–16 points depending on trunk size)
- Depth: 2–5 cm into xylem, depending on tree size
- Volume per point: Small, controlled doses (5–15 mL per point)
- Distribution: Evenly spaced around the trunk, avoiding clustering to prevent local stress
3. Preparation & Handling
- Mix carefully in clean water (follow label dilution instructions)
- Use dedicated tools and containers to avoid contamination
- Wear PPE: gloves, mask, goggles, long sleeves, closed shoes
- Do not mix with untested chemicals, fertilizers, or other microbes
4. Application Method
- Injection / drilling method: Deliver enzyme solution directly into xylem
- Ensure tight sealing of inoculation holes to prevent leakage
- Apply slowly and evenly to allow absorption
- Avoid over-application—too much enzyme can stress the tree
5. Post-Application Care
- Monitor resin formation weekly or monthly
- Avoid heavy irrigation or rainfall immediately after application
- Record application date, inoculant, and tree details for traceability
- Follow up with biotic inoculants if part of a dual or multi-step system
6. Environmental & Safety Considerations
- Store enzyme in cool, shaded, dry place
- Do not expose to direct sunlight for long periods
- Dispose of leftover enzyme solution safely, avoid water sources
- Keep away from children and livestock
7. Farmer-Friendly Summary
“Apply enzymes after priming chemicals, inject carefully into healthy trees, and let them open pathways for resin and fungi to do their job.”